Illumina Sequencing에서 사용되는
Adapter중 TruSeq (분석할때 받는 데이터들이 다 요녀석으로 되어 있어서...) DNA/RNA Adapter Sequence를 확인해서 확인해봤습니다. ㅎㅎ
Type
|
Sequence
|
TruSeq Universal Adapter
|
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
|
TruSeq Adapter, Index 1
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 2
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 3
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 4
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 5
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 6
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 7
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 8
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 9
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 10
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 11
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTG
|
TruSeq Adapter, Index 12
|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG
|
나중에 급할때 찾기 좀 애매해서...
요기다가 급 정리 ㅎㅎ
데이터 받았는데 TruSeq DNA/RNA Adapter인데 Index 12번보다 큰 경우
TruSeq Small RNA Index를 사용하는 것이라고 하네요
기본적인 Adapter sequence는 TruSeq DNA/RNA 인데 Index만 TruSeq Small RNA..
모 그렇다고 합니다. :)
좀더 자세한 Illumina Adapter Sequence에 대해서 알고 싶다면
다음 링크 참조 LINK
